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Genome editing

Expanded toolkits for RNA circularization

Nature Biomedical Engineering volume 9, pages 5–6 (2025)Cite this article

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Optimized methods for the synthesis of circular RNA in vitro and in cells, and a complementary Cas9 de-immunization method, enhance RNA persistence and reduce immunogenicity for applications in genome engineering and cell engineering.

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Fig. 1: Two RNA circularization strategies for facilitating genome editing and cell engineering.

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Author notes

  1. These authors contributed equally: Xiao Wang, Youkui Huang.

Authors and Affiliations

  1. Key Laboratory of RNA Innovation, Science and Engineering, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China

    Xiao Wang, Youkui Huang & Ling-Ling Chen

  2. ShanghaiTech University, Shanghai, China

    Ling-Ling Chen

  3. New Cornerstone Science Laboratory, Shenzhen, China

    Ling-Ling Chen

Authors
  1. Xiao Wang
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  2. Youkui Huang
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  3. Ling-Ling Chen
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Corresponding author

Correspondence to Ling-Ling Chen.

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Competing interests

L.-L.C. is a scientific co-founder of RiboX Therapeutics. All other authors declare no competing interests.

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Wang, X., Huang, Y. & Chen, LL. Expanded toolkits for RNA circularization. Nat. Biomed. Eng 9, 5–6 (2025). https://doi.org/10.1038/s41551-024-01262-y

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