Extended Data Fig. 9: Culturing of branching epithelial and mesenchymal organoids directly in 3D matrices. | Nature Biomedical Engineering

Extended Data Fig. 9: Culturing of branching epithelial and mesenchymal organoids directly in 3D matrices.

From: Heterogeneity-driven phenotypic plasticity and treatment response in branched-organoid models of pancreatic ductal adenocarcinoma

Extended Data Fig. 9

a Schematic representation of organoid generation directly from in vivo or 3D in vitro cultures (Matrigel), image created with BioRender.com. b Epithelial (top) and mesenchymal (bottom) collagen gel grown organoids. Corresponding HEs of the primary tumours. Confocal IF imaging of E-cadherin (green), Vimentin (red), DAPI (blue). Scale bars= 500 μm (bright field), 200 μm (HEs), 200 μm (IF).

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