Extended Data Fig. 4: Transcriptomic characterization of intestinal cells treated with microbiota from patients with melanoma. | Nature Biomedical Engineering

Extended Data Fig. 4: Transcriptomic characterization of intestinal cells treated with microbiota from patients with melanoma.

From: A gut-on-a-chip incorporating human faecal samples and peristalsis predicts responses to immune checkpoint inhibitors for melanoma

Extended Data Fig. 4: Transcriptomic characterization of intestinal cells treated with microbiota from patients with melanoma.

A) Reactome pathway analysis of NR vs CTR exclusive downregulated differentially expressed genes (DEGs), R vs CTR exclusive downregulated DEGs and common downregulated DEGs. B) Summary of enrichment analysis (coloured by p-values) of downregulated DEGs in TRRUST. All genes in the genome have been used as the enrichment background. Terms with a p-value < 0.01, a minimum count of 3, and an enrichment factor > 1.5 are collected and grouped into clusters based on their membership similarities. One term per cluster is shown in the figure. C) MYC expression (qPCR analysis) in untreated epithelial cells or epithelial cells treated with complete faecal material from faecal material of R and NR patients. Bars represent mean ± SD, two-tailed Welch’s test, p = 0.018 for CTR vs R, p = 0.0090 for CTR vs NR.

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