Fig. 4: Transcriptional profiling of the gut-on-a-chip response to faecal microbiota from patients with melanoma undergoing immunotherapy. | Nature Biomedical Engineering

Fig. 4: Transcriptional profiling of the gut-on-a-chip response to faecal microbiota from patients with melanoma undergoing immunotherapy.

From: A gut-on-a-chip incorporating human faecal samples and peristalsis predicts responses to immune checkpoint inhibitors for melanoma

Fig. 4: Transcriptional profiling of the gut-on-a-chip response to faecal microbiota from patients with melanoma undergoing immunotherapy.

a, Experimental design. Complete gut-on-a-chip were treated (day 8) with faecal material from patients with melanoma responsive (R, blue) or non-responsive (NR, red) to immunotherapy. b, PCA plot of untreated (CTR) and treated gut-on-a-chip with faecal material from responsive (R) patients and non-responsive (NR) patients. Dim1, dimension 1; Dim2, dimension 2. c, Sunburst plot showing the indicated number of DEGs, unique or shared (purple and light lime), in the gut-on-a-chip treated with whole faeces (Live, turquoise) or faecal supernatants (Sup, orange) from patients with melanoma that were responsive (blue) or non-responsive (red) compared with untreated controls (CTR). d, Reactome’s pathway analysis of NR versus CTR (red) and R versus CTR (blue) unique and common (purple) DEGs coloured by normalized enrichment score.

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