Extended Data Fig. 1: Human iPSC-derived intestinal organoids.

A) Schematic representation of the experimental plan. B) Characterization of mechanical actuation. Representative brightfield images of the Gut-on-Chip device in rest and actuated conditions. % Strain of the hydrogel walls (arrows) was calculated in both X and Y directions. C) Spatial distribution of proliferative cells (Ki-67) in static and actuated chips seeded with intestinal cells obtained from disaggregated human iPSCs-derived intestinal organoids (Day 9).