Extended Data Fig. 2: In vivo-produced tol-APCs mediate potent therapeutic effects in DSS-induced UC mice. | Nature Biomedical Engineering

Extended Data Fig. 2: In vivo-produced tol-APCs mediate potent therapeutic effects in DSS-induced UC mice.

From: Generation of tolerogenic antigen-presenting cells in vivo via the delivery of mRNA encoding PDL1 within lipid nanoparticles

Extended Data Fig. 2

a, Representative images of CD8, Foxp3, IFN-γ, and TNF-α staining from the colon of one mouse in a group of four. Arrows refer to Foxp3+ cells. Scale bar = 200 µm. b-e, The number of CD8+ (b) and Foxp3+ (c) cells and the percentage of IFN-γ+ (d) and TNF-α+ (e) area per FOV. Mice were treated with PBS, LNPs, or LNPs/mPDL1 (5 μg mRNA) via subcutaneous injection at the lower right back. Mice treated with cyclosporine served as the positive control group. Normal group comprises healthy mice. n = 4 biologically independent mice per group for data in b-e. Data are expressed as the mean ± s.e.m. Statistical significances were determined using one-way ANOVA with Dunnett’s post hoc test. Comparisons were performed between the LNPs/mPDL1 group and each of the other groups. N.S. is P ≥ 0.05, and significant P values are displayed.

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