Extended Data Fig. 2: The methods to prepare the PDMS stamp for the in vitro liver fibrosis model with the hexagonal liver lobule structure. | Nature Biomedical Engineering

Extended Data Fig. 2: The methods to prepare the PDMS stamp for the in vitro liver fibrosis model with the hexagonal liver lobule structure.

From: Tension-induced directional migration of hepatic stellate cells potentially coordinates liver fibrosis progression

Extended Data Fig. 2: The methods to prepare the PDMS stamp for the in vitro liver fibrosis model with the hexagonal liver lobule structure.

Key results are included in Fig. 2m. a, The blueprint of the PMMA (polymethyl methacrylate) mould with thin holes (A1). b, Preparation of the solid portal pillar (A2) by casting A1 with PDMS. c, The blueprint of the PMMA mould with thick holes (A3). d, Preparation of the hollow portal pillar (A4) by aligning A2 with A3, and casting with PDMS. e, The photomask (B1). f, Preparation of the photoresist pillar (B2) by lithography on glass substrate. g, Preparation of the PDMS pit (B3) by casting B2 with PDMS. h, Preparation of the PDMS pillar (B4) by casting B3 with PDMS. i, Preparation of the PDMS cover with through holes (B5) by smearing PDMS onto portal pillar tip of B4, crosslinking with PDMS substrate, and casting with PDMS. j, Preparation of the PDMS stamp (C) by aligning A4 with B5, and casting with PDMS. ‘P’ and ‘C’ represent the micro-structure corresponding to portal and central area in the in vitro model of liver fibrosis. j is partially created with BioRender.com. Engineering files of a, c and e can be accessed with the link in Supplementary Table 2.

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