Extended Data Fig. 2: Reduced fusion of organoids following XG treatment.
From: Scalable production of human cortical organoids using a biocompatible polymer
(a) Representative images of untreated hCO (top), xanthan gum-supplemented (hCO + XG, middle) and STEMdiffTM Neural Organoid Basal Medium 2 cultured hCO (hCO + STEMdiffTM NOBM, bottom). Scale bar: 5 mm. (b) Graph showing remaining organoids after 5 days of culture; n = 24 wells tested for untreated hCO, n = 24 wells tested for hCO + XG, n = 24 wells tested for hCO + ST from 2 differentiation experiments of 2 hiPS cell lines. Kruskal–Wallis test, ANOVA result: ****P < 0.0001, ****P < 0.0001 with multiple comparisons for untreated hCO versus hCO + XG, **P = 0.002 for hCO + XG versus hCO + ST. Data show mean ± s.e.m. (c) Immunostaining for SOX2 (yellow), PAX6 (magenta), and Hoechst (cyan) in hCO, hCO+XG, and hCO + ST at day 25. Scale bar: 200 µm, and 50 µm for an inset. (d) Quantified data of SOX2+ / Hoechst+ cells and PAX6+ / Hoechst+ cells in untreated hCO, hCO + XG and hCO + ST at day 25. n = 6 organoids for untreated hCO, n = 4 organoids for hCO + XG and n = 7 organoids for hCO + ST from 2 differentiation experiments of 1 hiPS cell line. Ordinary one-way ANOVA, P = 0.25 for SOX2+ / Hoechst+ cells and P = 0.70 for PAX6+ / Hoechst+ cells. Data show mean ± s.e.m. (e) Density of progenitor ventricular-like zones. n = 6 for hCO, n = 4 for hCO + XG, and n = 7 for hCO + ST. Ordinary one-way ANOVA, P = 0.37. Data show mean ± s.e.m.
