Extended Data Fig. 1: IVT, 5′ capped synthetic TERC RNA does not increase telomere length in TERC-null 293T cells by TRF analysis. | Nature Biomedical Engineering

Extended Data Fig. 1: IVT, 5′ capped synthetic TERC RNA does not increase telomere length in TERC-null 293T cells by TRF analysis.

From: Extension of replicative lifespan by synthetic engineered telomerase RNA in patient induced pluripotent stem cells

Extended Data Fig. 1

A, Top: Full length (451 nt) m7g-capped (Cap-0) synthetic IVT TERC RNA evaluated by agarose gel electrophoresis. n = 3 biological replicates, one representative shown. Bottom: Representation of IVT TERC with 5’Cap-0 (orange). B, TERC RNA by northern blot in TERC-null 293T cells compared to normal (WT) 293T cells, using 18S rRNA loading control. n = 3 biological replicates, one representative shown. C, Analysis of telomerase activity by TRAP assay in TERC-null 293T cells compared to normal (WT) 293T cells. n = 3 biological replicates, one representative shown. D, TRF analysis in TERC-null 293T cells compared to normal (WT) 293T cells. n = 3 biological replicates, one representative shown. E, TRF analysis in TERC-null 293T cells 96 h post-transfection of m7g-capped (Cap-0) synthetic TERC RNA and human TERT expressing plasmid vector. n = 3 biological replicates, one representative shown, compared to normal (WT) 293T cells. n = 3, one representative shown.

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