Extended Data Fig. 2: DNA matrix improve the delivery of DNA molecules and antigen.
a, Image of denature gel electrophoresis: main bands (blue rectangle), degraded bands (orange rectangle). The quantitative analysis of band intensity was shown. 10 μL DNA matrix, or DNA solution (Y) with equimolar amounts was incubated with an equal volume of FBS at 37 °C for 24 h or 72 h, respectively. Degradation status was measured by gel electrophoresis. b, Image of gold nanoparticle diffusion. c, Quantification of OVA retention in vitro. DNA matrix was mixed with OVA (100 μg), and PBS (100 μL) was added to the upper layer. OVA in supernatant was monitored. d, Quantification of total radiant efficiency of Cy5.5 in kidney, liver, and iLN. C57BL/6 mice were sacrificed at 12 h or 36 h after i.pl. injection with Cy5.5-labeled DNA solution (L) (1.07 mg) or Cy5.5-labeled DNA matrix (1.07 mg), respectively. Indicated organs were collected and subjected to an IVIS Spectrum system (n = 3/group). e-h, Detection of in vivo distribution of antigen. C57BL/6 mice (n = 3/group) were i.pl. injected with OVA-Cy5 (5 μg) alone, or together with DNA solution (Y) (1.07 mg) or DNA matrix (1.07 mg). Tissues were collected at 48 h.p.i. e, Representative IVIS images of pLN and quantification of total radiant efficiency of Cy5 in pLNs. f, Gating strategies of flow cytometry analysis for g and h. g, Flow cytometry analysis and percentages of OVA-Cy5+ cells in dLNs (n = 3/group) 48 h.p.i. h, Numbers of total OVA-Cy5+ cells, OVA-Cy5+ B cells, and OVA-Cy5+ DCs in dLNs (n = 3/group) 48 h.p.i. Data are shown as mean ± s.e.m. Statistical significance was determined by two-way ANOVA (d) or one-way ANOVA with Tukey correction (e, h). Data are representative of two independent experiments.
