Extended Data Fig. 4: DNA matrix induce efficient innate responses.

a-d, mRNA expression of IL-1β, CCL4, IL-6 and IFN-β in footpads were measured by RT-qPCR (n = 3/group). RNA was isolated from footpads of mice post i.pl. immunization with DNA matrix (1.07 mg) or Alum (1:1) at indicated time points. e, mRNA expression of IL-1β, CCL4, IL-6 and CCL2 in footpads was measured by RT-qPCR (n = 3/group). RNA was isolated from footpads of mice 12 h post i.pl. immunized with DNA matrix (1.07 mg), DNA solution (L) (1.07 mg), or DNA solution (Y) (1.07 mg) (n = 3/group). Data are shown as mean ± s.e.m. Statistical significance was determined by two-way ANOVA with Tukey correction (e). Data are representative of two independent experiments.