Extended Data Fig. 5: DNA matrix leads to LN expansion by recruiting circulating lymphocytes, and directly activates DCs. | Nature Biomedical Engineering

Extended Data Fig. 5: DNA matrix leads to LN expansion by recruiting circulating lymphocytes, and directly activates DCs.

From: Enantiomer-dependent and modification-free DNA matrix as an adjuvant for subunit vaccines against SARS-CoV-2 or pneumococcal infections

Extended Data Fig. 5: DNA matrix leads to LN expansion by recruiting circulating lymphocytes, and directly activates DCs.The alternative text for this image may have been generated using AI.

C57BL/6 mice were i.pl. immunized with DNA solution (Y) (1.07 mg) or DNA matrix (1.07 mg), respectively. 12 h or 36 h after injection, cells from popliteal LNs were harvested and subjected to flow cytometry analysis (n = 3/group). a, Total cell number of popliteal LNs were counted 12 h.p.i. b, Flow cytometry analysis of macrophages (F4/80+), B cells (B220+), and DCs (B220- F4/80- CD11c+ MHCII+) of pLNs 36 h.p.i. c and d, Flow cytometry analysis and quantification of resident DC and migratory DCs in pLN 36 h after DNA solution (Y) or DNA matrix injection. e, Flow cytometry analysis of MFI of CD86 in B cells (F4/80- B220+) in pLNs 36 h.p.i with Cy5.5 labelled DNA matrix. Data are shown as mean ± s.e.m. Statistical significance was determined by one-way ANOVA (a) or two-way ANOVA (d) with Tukey correction. Data are representative of three independent experiments.

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