Extended Data Fig. 6: DNA matrix induces quick and efficient innate responses in pLNs. | Nature Biomedical Engineering

Extended Data Fig. 6: DNA matrix induces quick and efficient innate responses in pLNs.

From: Enantiomer-dependent and modification-free DNA matrix as an adjuvant for subunit vaccines against SARS-CoV-2 or pneumococcal infections

Extended Data Fig. 6: DNA matrix induces quick and efficient innate responses in pLNs.The alternative text for this image may have been generated using AI.

a-d, mRNA expression of IL-1β, CCL4, IL-6 and IFN-β in pLNs were measured by RT-qPCR (n = 3/group). RNA was isolated from pLNs of mice post i.pl. immunization with DNA matrix (1.07 mg) or Alum (1:1) at indicated time points. e, Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis of upregulated DEGs in DNA matrix group compared with PBS control. f and g, mRNA expression of IL-6 and IL-12b in BMDCs were measured by RT-qPCR (n = 3/group). BMDCs were pretreated with or without R406 (2.5 μM) for 30 min, and then stimulated with or without DNA matrix (10 μg/mL) for another 6 hours. Data are shown as mean ± s.e.m. Statistical significance was determined by two-way ANOVA with Tukey correction (f, g). Data are representative of two independent experiments.

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