Extended Data Fig. 10: DNA matrix induced higher antibody response in a multiple CG sequences-dependent manner.
a, mRNA expression of indicated genes in footpad (n = 3/group). b and c, The molecular weight and sequence information of ssDNA in DNA matrix-12 and DNA matrix-56, respectively. Purple colored sequences are sticky ends, and red colored sequences are sequences of CpG 1018 and poly (dA:dT). d, mRNA expression of IL-6, IL-1β and CCL4 in BMDCs was measured by RT-qPCR (n = 3/group). BMDCs were stimulated with 1 mg / mL DNA matrix, DNA matrix-12, or DNA matrix-56 for 6 hours. e, Schematic diagram of inversion CG to GC in Y1 and Y2 ssDNA to form GC-DNA matrix. f, The molecular weight and sequence information of ssDNA in GC-DNA matrix. Purple colored sequences are sticky ends and yellow highlighted parts are single GC-swapped sequences resembling known CpG sequence. g, Schematic diagram of experimental design for Fig. 8l. C57BL/6 mice were i.pl. immunized with OVA (100 μg) alone, or together with original DNA matrix (CG) or GC-DNA matrix on day 0. Sera were collected on day 14. h, The molecular weight and sequence information of ssDNA in tGC-DNA matrix. Red colored sequences are GC-swapped positions. i, OVA-specific IgG titers in sera were measured (n = 6/group). C57BL/6 mice were i.pl. immunized with OVA (100 μg) alone, or together with DNA matrix (300 μg) or CpG DNA (PD) (300 μg) in a total volume of 10 μL on days 0. Sera were collected on day 14 for ELISA. Data are shown as mean ± s.e.m. Partial elements were created in BioRender.com (e, g). Statistical significance was determined by one-way ANOVA (i) or two-way ANOVA (a) with Tukey correction. Data are representative of two independent experiments.
