Extended Data Fig. 6: Amph-mimotope labeling of APCs and stimulation of FMC63-mCAR-T cell expansion in vivo. | Nature Biomedical Engineering

Extended Data Fig. 6: Amph-mimotope labeling of APCs and stimulation of FMC63-mCAR-T cell expansion in vivo.

From: Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells

Extended Data Fig. 6

(a) Gating strategies and surface makers used for defining APC populations in the lymph node. (b) C57BL/6 mice (n = 5 animals/group) were lymphodepleted (L.D.) with 500 cGy gamma irradiation, adoptively transferred with 106 FMC63-mCAR-T cells, and then vaccinated at indicated time points. Shown are the number of circulating FMC63-mCAR-T cells per microliter of blood quantified by flow cytometry over time. This data is related to Fig. 5e. Error bars show mean ± 95% CI. ***, p < 0.0001 by two-way ANOVA with Tukey’s post-test.

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