Extended Data Fig. 2: Translation and transcription of IL-12 variants in Jurkat and primary mouse T cells. | Nature Biomedical Engineering

Extended Data Fig. 2: Translation and transcription of IL-12 variants in Jurkat and primary mouse T cells.

From: Collagen-binding IL-12-armoured STEAP1 CAR-T cells reduce toxicity and treat prostate cancer in mouse models

Extended Data Fig. 2: Translation and transcription of IL-12 variants in Jurkat and primary mouse T cells.

(a) Jurkat cells transduced with EGFP + NFAT-CBD-IL-12-CBD gamma-retroviral vector were stimulated with PMA/Iono in the presence of Brefeldin A followed by intracellular staining of mouse IL-12. Representative contour plots of n = 2 technical replicates. (b) Primary mouse T cells transduced with the indicated gamma-retroviral vectors were stimulated with PMA/Iono in the presence of Brefeldin A followed by intracellular staining of mouse IL-12. Representative contour plots of n = 2 technical replicates. (c) Primary mouse T cells transduced with the indicated gamma-retroviral vectors were stimulated with PMA/Iono followed by RNA extraction, reverse transcription and quantitative PCR. Expression levels of mouse p35 and p40 in PMA/Iono stimulated cells relative to unstimulated cells were shown (n = 2 technical replicates).

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