Extended Data Fig. 4: Detection of IL-12 from tumour and organs, related to Fig. 3.
(a) Background concentration of IL-12 in major organs from RM9-hSTEAP1 tumour-bearing mice treated with 10 million UTD T cells (organs were collected when tumour volume reached the endpoint), normalized by total tissue weight (each dot represents a mouse, mean ± s.e.m.). (b) FVB/N male mice bearing subcutaneous MyC-CaP-hSTEAP1 tumours (200–270 mm3) were intravenously injected with 5 million CAR-T cells. Tumours were collected after 4 days and intratumoural IL-12 concentration was quantified by ELISA and normalized by total protein content (n = 2 tumours × 3 fragments = 6 replicates for each group, mean ± s.e.m.). Statistical analysis was performed using (b) two-tailed Welch’s t-test. P values are shown in figures.
