Fig. 3: Effect of RNA Staple oligomer on TRPC6 gene expression in mice.
a, A schematic of the recombinant AAV6 vector genome encoding the Staple oligomer. A short hairpin RNA expression vector was used to express the RNA Staple oligomer. The U6 promoter, Staple oligomer and inverted terminal repeat (ITR) are shown in black, red and grey, respectively. AAV6 infection was confirmed by ZsGreen expression with CMV promoter. The RNA Staple oligomer is expressed by the AAV6 expression system in target organs. AAV6 infects the heart and liver, but not the kidney. b, Evaluation of protein expression levels of TRPC6 in each organ by western blotting. TRPC6 expression was suppressed by 70–80% in the heart and liver by Staple oligomers, but not in the kidney, which is consistent with the infection directedness of AAV6. c, Evaluation of TRPC6 mRNA expression levels in each organ by qPCR. No appreciable change in the mRNA expression levels was observed in each organ by the introduction of RNA Staple oligomers, clearly supporting the mechanism of action of RNAh that can suppress TRPC6 expression without mRNA degradation. Data are mean ± s.d. of three independent experiments.
