Extended Data Fig. 5: p239:NB-5R nanoparticles induced immune response against SARS-CoV-2 in mice and hamster.
a p239:NB-5R nanoparticles induced cross-reactive antibodies response in mice. End-point ELISA data for day 56 (14 days after the 2nd boost) for antisera from individual mice showing the maximal dilution titer. b p239:NB-5R nanoparticles induced cross-neutralizing antibodies response in mice. In the box‑and‑whisker plots, the plus sign indicates the median, the top and bottom of the box the interquartile range, and the whiskers the range. Symbols represent individual animals. Dashed lines indicate the assay limit of detection. c End-point ELISA data for prime and boost for antisera showing the maximal dilution titer. (d-e) p239:NB-5R nanoparticles induced cross-neutralizing antibodies response in hamsters for day 28 (d) and 56 (e) (14 days after the 1st and 2nd boost). Antisera from individual hamster showing the geometric mean titers (GMT), presented as geometric mean with standard deviation (SD). H, 10 µg; L, 2 µg dosage. Saline, placebo cohort. The vaccination experiments above were conducted once. The end-point ELISA and neutralization assays were conducted more than twice. Statistical analysis was performed by Kruskal-Wallis test with Dunn’s multiple comparisons.
