Extended Data Fig. 5: Important features associated with cleavage ratio index differences. | Nature Biomedical Engineering

Extended Data Fig. 5: Important features associated with cleavage ratio index differences.

From: High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants

Extended Data Fig. 5

The 15 most important features associated with cleavage ratio index differences for HF1 (a), NRRH-HF1 (b), or NRCH-HF1 (c) were determined by Tree SHAP after considering multicollinearity, using a threshold of 0.7 for Pearson correlation coefficients. A high SHAP value indicates that the feature is associated with a high cleavage ratio index difference. Red and blue dots respectively represent high and low values of the relevant feature. Tm, melting temperature; GN19, sgRNA spacer starts with G. The number of sgRNA and target sequence pairs (that is, the number of dots per feature in the summary plot) (n) = 62,397 for HF1, 64,461 for NRRH-HF1, and 63,599 for NRCH-HF1.

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