Extended Data Fig. 8: Optimal conditions for selective enrichment of rare variant sequences. | Nature Biomedical Engineering

Extended Data Fig. 8: Optimal conditions for selective enrichment of rare variant sequences.

From: High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants

Extended Data Fig. 8

a-c, The effects of different HF1:sgRNA molar ratios (a), HF1 concentrations (b), and cleavage reaction times (c) on the fold enrichment (that is, VAF fold increases). Only a single cleavage reaction cycle was conducted using the optimized_HF1_2k sgRNA library and a 1,000: 1 mixture of 7.8k_WT and 7.8k_MT. The boxes represent the 25th, 50th, and 75th percentiles; whiskers show the 10th and 90th percentiles. The number of target mutations for each condition (n) = 2,612.

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