Extended Data Fig. 2: Development of Cut-seq2. | Nature Biomedical Engineering

Extended Data Fig. 2: Development of Cut-seq2.

From: High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants

Extended Data Fig. 2

a, The structures of vectors used for Cut-seq1 and Cut-seq2. EcoRV, EcoRV restriction enzyme site; Constant, a constant sequence for identifying reads uncut by Cas9; UMI, unique molecular identifier; T7, T7 promoter; T7-term, T7-terminator; EcoR1, EcoR1 restriction enzyme site. b, Schematic representation of Cut-seq2. Illustrations in b created with BioRender.com.

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