Extended Data Fig. 3: In vitro cleavage activities of Cas9 measured using Cut-seq2. | Nature Biomedical Engineering

Extended Data Fig. 3: In vitro cleavage activities of Cas9 measured using Cut-seq2.

From: High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants

Extended Data Fig. 3

a, Correlations between cleavage ratio indices of two biological replicates. The Pearson correlation coefficient (r) and Spearman correlation coefficient (R) are displayed. The number of sgRNA and target pairs (n) = 25,644 for HF1, 27,260 for NRRH-HF1, and 25,553 for NRCH-HF1. b, Heatmaps showing the effect of the number of mismatched nucleotides, DNA bulges, or RNA bulges in the sgRNA relative to the target on the mean cleavage ratio indices of Cas9 variants measured using Cut2_30k and Cut2_42k. HF1, SpCas9-HF1; NRRH-HF1, SpCas9-NRRH-HF1; NRCH-HF1, SpCas9-NRCH-HF1.

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