Extended Data Fig. 6: Infusion of kidney organoid-derived cells during ex vivo NMP in porcine kidneys.
a,b, Photographs of the homemade NMP setup used during porcine kidney perfusion. c, Arterial flow (mL/min/100 g) of kidneys infused with organoid disaggregated cells during NMP. The yellow arrow indicates the time of organoid infusion. d, Sodium concentration (mmol/L). e, Fractional excretion (FE) of sodium (FENa+) (%) and area-under-the curve (AUC). f, Creatinine clearance (mL/min/100g) and AUC. g, Lactate dehydrogenase (LDH) levels (U/L) and AUC. h, Aspartate aminotransferase (ASAT) levels (U/L) and AUC. In c-h, Data are mean ± s.d. n= 2. i, Detection of Alu repeat sequences of human DNA present in formalin fixed paraffin sections of porcine renal cortex by in situ hybridization. Images correspond to kidneys after infusion of disaggregated kidney organoid cells during ex vivo NMP. Scale bars, 50μm, 25μm (magnified views). n = 3 cortical punch biopsies were analyzed from two independent experiments, with similar results. j, Detected Qdot-positive cells (pseudocolored in red) and rendered along with brightfield volume of a 400 μm-thick slice from one kidney infused with dissociated kidney organoid cells.
