Extended Data Fig. 1: The LDH exhibits favorable physicochemical properties and can protect neurons in enTsOrg under hypoxic conditions.
From: Engineered thoracic spinal cord organoids for transplantation after spinal cord injury
a, Diagram of the Mg/Fe-LDH and LDH-Matrigel preparation. b, XPS spectrum of Mg/Fe-LDH. c, XRD spectrum of Mg/Fe-LDH. d, CD spectra of Matrigel (black line) and LDH-Matrigel (red line). e, Representative SEM images of LDH (left), Matrigel (middle) and LDH-Matrigel (right). f, TUNEL analysis and quantification results of cell apoptosis under hypoxic conditions at day 21. Two-sided Unpaired t-test (n = 5 in OGD group; n = 6 in Ctrl group, biological replicates). Data represent the mean ± s.e.m. (f). Scale bars, 200 μm in (f). LDH, layered double hydroxides; XPS, X-ray photoelectron spectroscopy; XRD, X-ray Diffraction; CD, circular dichroism; SEM, scanning electron microscope; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; ns, no significant difference.
