Extended Data Fig. 2: The safety analysis of enTsOrg and sOrg for transplantation.
From: Engineered thoracic spinal cord organoids for transplantation after spinal cord injury
a, Photograph of spinal cord morphology at 1, 3, 5, 7 and 14 weeks post-transplantation. b, Quantification of graft area in sOrg and enTsOrg at 1, 3, 5, 7 and 14 weeks post-transplantation. Two-way ANOVA with Tukey’s multiple comparisons test was used (n = 4 at 1, 3 wpi; n = 2 at 5 wpi; n = 5 at 7 wpi; n = 3 at 14 wpi, biological replicates). c, Immunofluorescence staining for CEA in spinal cords after organoid transplantation. d, Survival proportions of M-NSG mice after surgery within 7 weeks. e, Plot of weight change in different treatment groups (n = 20-22 at pre-7 wpi each group; n = 3-7 at 8-14 wpi each group, biological replicates). Data represent the mean ± s.e.m. (e). Scale bars, 200 μm in (c). SCI, spinal cord injury; CEA, Carcino-embryonic antigen; wpi, weeks past injury; ns, no significant difference.
