Extended Data Fig. 6: TOBis MC cell line preparation and preliminary analysis. | Nature Biomedical Engineering

Extended Data Fig. 6: TOBis MC cell line preparation and preliminary analysis.

From: Human gastric multi-regional assembloids for functional parietal maturation and patient-specific modelling of antral foveolar hyperplasia

Extended Data Fig. 6

a, Plasmid maps and generation of reporter RFP-fundus and GFP-body organoid lines by electroporation. Scale bars 200 μm. b, 2nd and 3rd selections of electroporated GOs at day 7 of passage (top). Fusion of adjacent regions in MRA (bottom). RFP-expressing fundus organoids, GFP-expressing body organoids. Scale bars 200 μm. c, Optimisation of antibody staining of MRA tubes for CyTOF.

Back to article page