Extended Data Fig. 8: MRA functional analysis.
a, Immunofluorescence of sectioned antrum tissue showing ATPase β in magenta, and Nuclei (Hoeachst) in cyan. Scale bar 200μm. b, Immunofluorescence of sectioned fundus tissue showing ATPase β in magenta, and Nuclei (Hoeachst) in cyan. Scale bar 200μm in the first panel, 50μm in the following. c, Timelapse frames of Acridine orange staining (10 μM) of Fundus, Body, and Antrum in MRA, Control, Histamine treatment (100 μM), Histamine+Omeprazole (100 μM). Images acquired every 10 mins. d, Acridine orange 650/550 nm ratio quantification of Fundus, Body, and Antrum in SRA, normalized to MRA Antrum-control. Control (black), Histamine treatment (100 μM, red), Omeprazole treatment (100 μM, orange), Histamine+Omeprazole (100 μM, green). Circles indicate average of single patient data points, mean ± SD (n = 4, 2 patients). One-way ANOVA, NS. e, Acridine orange 650/550 nm ratio timelapse quantification of single cells from Fundus, Body, and Antrum in SRA and MRA at 6 timepoints (every 10 min for 1 h). Control (circles), Histamine treatment (100 μM – triangles), Omeprazole (100 μM – rombi), Histamine+Omeprazole (100 μM – squares). Data points indicate average of 12 to 31 cells per each point. mean ± SD (2 patient). One-way ANOVA of t5 timepoint (for single p-value, refer to the source data for Fig. 4j). f, Acridine orange 650/550 nm ratio timelapse quantification of single cells in SRA (black filled symbols) and MRA (coloured symbols) at 6 timepoints (every 10 min for 1 h) subdivided in three graphs per region: Antrum (orange), Body (green), and Fundus (blue). Control (circles), Histamine treatment (100 μM – triangles), Omeprazole (100 μM – rombi), Histamine+Omeprazole (100 μM – squares). Data points indicate average of 12 to 31 cells per each point. mean ± SD (2 patient).
