Extended Data Fig. 9: In vivo implantation of single- and multi-regional gastric assembloids.
a, Schematic representation of assembloid implantation workflow. b, Postimplantation assembloid harvesting: sub-cutaneous location (left), after explanation (central, scale bar 5 mm), isolated assembloid (right, scale bar 500 μm) (n = 3, patient 1). c, Microfocus computed tomography (Micro-CT) of transplanted SRA from axial and coronal view. Scale bar 500 μm. d, H&E staining of iMRA slice. Scale bar 500 μm. e, Whole mount 3D reconstruction of gastric iMRA showing nuclei in white (Reddot), Mucin 5AC (MUC5AC) in magenta. Scale bar 100 μm. f, Principal component analysis (PCA) of RNA-sequencing samples from Fundus (blue), Body (green), and Antrum (red) in MRA (triangle) and postimplantation MRA (iMRA, inverted triangle). (n = 3, patient 1). g, Hierarchical clustering of DEGs (FDR < 0.05, fold change > 1.5) between Antrum and Fundus in MRA (left), and expression of the corresponding genes in iMRA (right). (n = 3, patient 1). Color bar refers to centered log2(CPM + 1). h, Heatmaps of selected gastric specific genes differential expression between Antrum and Fundus in MRA or in iMRA. (n = 3, patient 1). Panel a created with BioRender.com.
