Fig. 2: Gastric assembloid generation and characterization. | Nature Biomedical Engineering

Fig. 2: Gastric assembloid generation and characterization.

From: Human gastric multi-regional assembloids for functional parietal maturation and patient-specific modelling of antral foveolar hyperplasia

Fig. 2

a, Schematic of SRA and MRA generation. b, Brightfield image of organoid fusion at day 4 of floating collagen I hydrogel culture. Scale bar, 200 μm. c, Brightfield image of an SRA at 10 days. Scale bar, 1 mm. d, Whole-mount imaging of a gastric SRA from the luminal side, showing E-cadherin (E-cad) in magenta. The yellow squares highlight an aperture in the lumen and a bud-like protrusion emerging from the lumen. Scale bar, 100 μm. e, SRA section stained for nuclei (white; RedDot) and MUC5AC (magenta). Scale bar, 50 μm. f, Whole-mount images of gastric SRAs, showing INT-β4 (red) and F-actin (F-act; cyan). The image was processed with Imaris. Scale bars, 500 μm (main image) and 100 μm (inset). g, Brightfield image of an MRA at 10 days. Scale bar, 1 mm. h, TEM images of MRAs. The grey circles within the cells indicate mucin-filled granules. The square, asterisks and arrowheads represent a gland, microvilli and desmosomes, respectively. Scale bars, 2 μm. i, Whole-mount immunofluorescence image of a gastric MRA, showing nuclei (blue; Hoechst), somatostatin (SST; green), CHGA (red) and PDX1 (orange) or nuclei (blue; Hoechst), MUC5AC (green), PGC (red) and PDX1 (orange). The central column provides an overview of the whole tube structure (scale bar, 500 μm) whereas the left and right columns show high magnification (scale bars, 50 μm). Panel a created with BioRender.com.

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