Extended Data Fig. 1: Tissue regionality and organoid characterization and assembloid protocol generation set-up.
a, Immunofluorescence panel showing Fundus and Antrum in paediatric stomach tissue sections stained for ATPase H+/K+ transporting subunit β (ATPase β) in cyan, Gastrin (GAST) in yellow, Pancreatic and Duodenal homeobox 1 (PDX1) in magenta, and nuclei in grey (Hoechst); or Integrin-β4 (INT-β4) in cyan, Pepsinogen C (PGC) in yellow, Ghrelin (GHRL) in magenta, and nuclei in grey (Hoechst). Scale bar 25 μm. b, Immunofluorescence panel showing foetal stomach (post-conception week 10) tissue sections stained for Integrin-β4 (INT-β4) in green, Pancreatic and Duodenal homeobox 1 (PDX1) in magenta, and nuclei in blue (Hoechst). Scale bar 250 μm. c, PCA of RNA-sequencing samples from Fundus (blue), Body (green), and Antrum (red) tissue (circles) versus organoids (squares). (n = 3, patient 1). d, Expression of typical gastric markers in tissue and organoids in Fundus (blue), Body (green), and Antrum (red). Circles indicate single data points, error bar indicates mean ±SD (n = 3, patient 1). CPM: counts per million. e, Custom-designed well for assembloid seeding: mould design (left), 3D printed mould (top right), PDMS customized-well (bottom right). f, SRA formation at various concentrations of collagen I hydrogel (0.75, 2, or 4.5 mg/ml). Scale bar 2 mm.
