Fig. 4: Expression of the incompatible CD4 co-receptor in cytotoxic T cells enhances ligand discrimination. | Nature Biomedical Engineering

Fig. 4: Expression of the incompatible CD4 co-receptor in cytotoxic T cells enhances ligand discrimination.

From: Generation of T cells with reduced off-target cross-reactivities by engineering co-signalling receptors

Fig. 4

a, Left: schematic of CD4 expression in cytotoxic T cells and flow cytometry staining of CD4 expression. Right: schematic of CD8→CD4 co-receptor switch T cells and flow cytometry staining of CD4 and CD8 expression. b, U87 cells were titrated with each of the 7 NY-ESO-1 peptides to stimulate WT or CD4-expressing cytotoxic T cells (top), or WT or CD8→CD4 co-receptor switch cytotoxic T cells (bottom). Target killing was measured after 20 h. c, The fold change in potency (P15) between the indicated modified and WT cytotoxic T cells over TCR–pMHC affinity (KD). Data for CD8 KO are shown from Fig. 1. Data in a and b are representative of N = 3 independent experiments with different blood donors. Data in c are shown as mean ± s.d. of N = 3 independent experiments with different blood donors. P values were determined using a two-tailed F-test. *P < 0.05, ****P < 0.0001.

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