Fig. 7: CD8→CD4 co-receptor switch cytotoxic T cells display reduced cross-reactivity to self-peptides. | Nature Biomedical Engineering

Fig. 7: CD8→CD4 co-receptor switch cytotoxic T cells display reduced cross-reactivity to self-peptides.

From: Generation of T cells with reduced off-target cross-reactivities by engineering co-signalling receptors

Fig. 7

a, U87 cells were titrated with each of the predicted self-peptides to stimulate WT or CD8 KO cytotoxic c259 TCR-T cells (top), or WT or CD8→CD4 co-receptor switch cytotoxic c259 TCR-T cells (bottom). Target killing was measured after 20 h. b, Fold change in P15 between modified and WT T cells from a is plotted over TCR–pMHC affinity (KD). Data are shown as mean ± s.d. of N = 3 independent experiments with different blood donors. P values were determined using two-tailed F-test. c, P15 from a is plotted for the indicated peptides. Each data point represents an independent experiment. P values were determined using one-way ANOVA and Tukey’s multiple-comparisons test. Data in a are representative of N = 3 independent experiments with different blood donors. *P < 0.05, **P < 0.01, ***P < 0.001.

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