Extended Data Fig. 2: The impact of different T cell co-signalling receptors on ligand sensitivity and discrimination using target cell killing.
(A) Flow cytometry staining of WT cells (Black) or KO T cells (Red) using knock-out target antibodies, a 9V HLA-A*02:01 tetramer or an anti-TCR Vβ13.1 antibody. (B) U87 cells were titrated with each of the 7 NY-ESO-1 peptides to stimulate WT or KO c259 TCR-T cells. Killing of the target U87 cells was measured after 20 hours. Dashed line indicates potency (P15). (C) Change in potency over affinity as described in Fig. 1d. Data in (A) and (B) are representative of at least N=2 independent experiments with different blood donors. Dashed line in (C) indicates fold change of 1. Data in (C) is shown as means ± SDs. Significance of non-zero slope was assessed by a two-tailed F-test.
