Fig. 2: HP induces MSC death in a lysosome-dependent manner.
a, Venn diagram showing overlapping proteins between lysosome-associated proteins (LAP) and upregulated hydrolase function proteins in the HP group vs the PP group. b, Clustering heat map analysis showing 199 highly expressed hydrolase function proteins in the HP group vs the PP group. c, Venn diagram showing overlapping proteins between LAP and upregulated proteolytic process proteins in the HP group vs the PP group. d, Clustering heat map analysis showing 59 highly expressed proteolytic process proteins in the HP group vs the PP group. e, Western blotting analysis of LDCD-related molecule expression after HP treatment for different periods of time. f, Representative super-resolution SIM microscopy images of calpain 2 and LAMP2 expression, and spatial distribution after HP treatment at different times. siRNA, calpain 2 siRNA treatment. Scale bar, 500 nm. g, Semi-quantification analysis of calpain 2, LAMP2 expression, co-localization between calpain 2 expression and LAMP2 expression, and the size of lysosomes in MSCs after HP treatment at different times. h, Representative super-resolution SIM microscopy images (top) and semi-quantification analysis (bottom) of LysoTracker in human umbilical cord MSCs after HP treatment at 24 h. Scale bar, 10 μm. i, Representative super-resolution SIM microscopy images of AO staining (left) and semi-quantification curves of kinetic plots of AO staining versus time (right) in human umbilical cord MSCs treated with HP. Scale bar, 10 μm. Error bars are means ± s.d. n = 3 (e) or 10 (f–i) biological repeats for each group. Data were analysed using one-way ANOVA with Tukey’s correction.
