Extended Data Fig. 3: Immunohistochemical analysis of lung-cancer associated markers in primary tumours and overgrown cultures.
From: Lung tumouroids as a testing platform for precision CAR T cell therapy
a+b, IHC staining for lung cancer-associated markers in adenocarcinoma primary tumour samples (TT11, TT24) and overgrown cultures (011, 024). Positive antibody staining is shown in brown, with hematoxylin counterstaining of nuclei in blue. In contrast to the primary tumours (TT11, TT24), which show TTF1 + , p40-, p63-, CK5/6-, CK7+ patterns, the overgrown cultures (011, 024) resembled the TTF1 mixed, p40 + , p63 + , CK5/6 + , CK7+ pattern of healthy organoids (HO) and bronchial epithelial cells from healthy tissues (HT, b). IHC staining was performed on n = 1 TO passage and n = 1 TT tissue section. Scale bars, 100 µm. c, Heatmap displaying histoscores (H-scores) of IHC stainings.
