Extended Data Fig. 8: Screening optimized forms of PE editors for T cell engineering.
a, The constructs of various promoters driving the expression of intein-split PEmax in primary T cells. b and c, Delivery efficiencies (b) and expression intensities (c) after electroporation with 2 μg of plasmids expressing PEmax-Cpart-T2A-mCherry driven by various promoters in primary T cells (from n = 3 independent biological donors). For b and c, The results are presented as the mean ± SEM. Statistical analyses were done using one-way ANOVA with Dunnett’s multiple comparisons test. ns, no significance. d, Schematics of split-intein PEmax used in PRIME-In 2.0-mediated targeted KI. e, Editing efficiencies of PRIME-In 2.0-mediated targeted KI with various intein-split PEmax constructs at AAVS1 locus in HEK293T cells. KI efficiencies were measured as the percentage of EGFP+ cells among successfully transfected cells. Three independent biological replicates were performed. The results are presented as the mean ± SD. Statistical significance was calculated using one-way ANOVA with Dunnett’s multiple comparisons test. ns, no significance.
