Extended Data Fig. 6: DEL selection against the DNA-tagged CA-12 on live cells.
From: Selection of DNA-encoded chemical libraries against endogenous membrane proteins on live cells
a) Structures of GLCBS–DNA and CBS–DNA, which were two positive controls added to the 4,800-member DEL for selection against CA-12 on A549 cells. b) Scatter plots of the selection results of the tagged A549 cells (top) and the untagged cells (bottom). The selection experiment condition and data processing protocol are the same as in Fig. 7. x-axis: post-sequencing counts; y-axis: enrichment fold = (post-selection %)/(pre-selection %) of each compound. The positive controls (GLCBS and CBS) are highlighted. c) Calculation of the DNA-tagged CA-12 concentration on A549 cells. The average number of DNA on each cell was determined with flow cytometry.
