Extended Data Fig. 1: Labelling of CA-12 on live cells using CBS-guided BP/CP probes.
From: Selection of DNA-encoded chemical libraries against endogenous membrane proteins on live cells
a) Structures of CBS-BP, NC-BP (a negative binding probe), and CP-1. A549 cells were labelled with CBS-BP/CP-1 and NC-BP (no CBS)/CP-1, respectively, targeting CA-12 on the cell surface. Experimental conditions are the same as in Fig. 2 of the main text. b)-c) Flow cytometry analysis of the labelled A549 cells. d) Column graph summarizing the flow cytometry results. e) CBS-BP and NC-BP were paired with biotin-CP-1, respectively, and used to label CA-12 on A549 and MCF-7 cells. After labelling, the cells were lysed and the biotinylated proteins were analysed with western blotting. M: marker; lanes 1 and 3: with CBS-BP; lanes 2 and 4: with NC-BP. *: endogenous biotinylated proteins. Loading control: internal endogenous biotinylated proteins, marked with *; a portion of each sample was separately blotted for CA-12 and actin as additional input/sample processing controls. In d), n = 3 biologically independent samples were measured; data are presented as mean values ± SD (standard deviation).
