Extended Data Fig. 3: Directed evolution of DA7.
From: Efficient Lewis acid catalysis of an abiological reaction in a de novo protein scaffold
a, Simplified schematic showing the steps of computational design and directed evolution leading to DA7. b, Amino acid alignment of evolutionary parent DA0, evolved variant DA7, and two intermediate variants, DA1 and DA4. Mutations distinguishing the variants are highlighted in orange. The two mutations suggested by computation are shown in pink.
