Extended Data Fig. 4: In vitro reaction analysis of MaMO and MaDA.
From: FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis
When MaDA was incubated with 3 alone, no new peak appeared, which revealed the MaDA did not have the oxidative function. In contract, when MaMO was used, diene 4 was formed. On the other hand, when MaMO was incubated with 2 and 3, 3 was completely transformed to diene 4, but no chalcomoracin or oxidative product of 2 was observed which indicated MaMO neither oxidized 2 nor catalysed the [4 + 2] cycloaddition between 2 and 4. The experiments were repeated three times independently with similar results.
