Extended Data Fig. 5: Effects of pH, temperature and divalent metal ions on the activity of MaMO and MaDA.
From: FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis

a, Effect of temperature on MaMO’s activity. b, Effect of divalent metal ions on MaMO’s activity. c, Effect of pH value on MaMO’s activity. d, Effect of temperature on MaDA’s activity. e, Effect of divalent metal ions on MaDA’s activity. f, Effect of pH value on MaDA’s activity. CK means ‘control check’. Enzyme activity values represent mean ± standard deviation (s.d.) of three independent replicates.