Extended Data Fig. 6: The time course for the incubation of morachalcone A (2) and 4 without MaDA.
From: FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis
The reaction mixture containing 20 mM Tris-HCl, pH 8.0, 100 μM morachalcone A (2), 100 μM diene (4) as substrates without MaDA in a final volume of 100 μL was incubated at 50 °C for 100 min. The reactions were terminated by the addition of 200 μL of ice-cold MeOH and were centrifuged at 15,000 g for 30 min. The supernatants were analysed by the LC/MS. The experiments were repeated three times independently with similar results.
