Extended Data Fig. 7: Determination of the kinetic parameters of MaDA using stable diene 10 and morachalcone A (2).
From: FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis
a, Enzymatic assay of 2 and 10 with MaDA. Compounds 2 and 10 were incubated with 270 nM MaDA or boiled MaDA for 5 min. The experiments were repeated three times independently with similar results. b, Kinetic parameters of MaDA for 2 (5–300 μΜ) using 10 (1.5 mM) as diene. c, Kinetic parameters of MaDA for 10 (5–800 μΜ) using 2 (4 mM) as dienophile. KM, kcat and kcat/KM values represent mean ± standard deviation (s.d.) of three independent replicates.
