Extended Data Fig. 5: Intact protein mass spectrometry demonstrating TReND reactivity with other Fic enzymes. | Nature Chemistry

Extended Data Fig. 5: Intact protein mass spectrometry demonstrating TReND reactivity with other Fic enzymes.

From: Identification of targets of AMPylating Fic enzymes by co-substrate-mediated covalent capture

Extended Data Fig. 5

LC/ESI-MS measurements of strategically placed cysteine mutants of Fic enzymes such as a, Bartonella hanselea BepA b, human HYPE/FicD c, Legionella pneumophila LpFic and d, Legionella pneumophila LtpG incubated with TReNDs. Corresponding cysteine modification and reactive TReND is indicated. For all panels: Whenever a representative result is shown, each experiment was repeated independently at least twice with similar results.

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