Extended Data Fig. 8: BepA cysteine mutants and their reactivity with various TReNDs.
From: Identification of targets of AMPylating Fic enzymes by co-substrate-mediated covalent capture
PhosTag SDS-PAGE shows that only BepA S198C and N111C mutants reacted with TReNDs. BepA S198C mutant showed better reactivity towards (1) and lesser reactivity with (3). BepA N111C showed overall good reactivity with all the TReNDs. Supported with the MS data in Supplementary Fig. 5a, BepA N111C was chosen as the optimal cysteine substitution mutant. Whenever a representative result is shown, each experiment was repeated independently at least twice with similar results.
