Extended Data Fig. 1: Schematic representation of the co-substrate-mediated covalent capture workflow for target identification.
From: Identification of targets of AMPylating Fic enzymes by co-substrate-mediated covalent capture
Fic enzymes with affinity tags are used to capture their putative targets in mammalian lysates by forming a covalent ternary complex. Those covalent ternary complexes are then enriched by affinity methods and subsequently subjected to tryptic digestion and LC-MS/MS for target identification.
