Extended Data Fig. 3: Intact protein mass spectrometry demonstrating TReND-6 reactivity. | Nature Chemistry

Extended Data Fig. 3: Intact protein mass spectrometry demonstrating TReND-6 reactivity.

From: Identification of targets of AMPylating Fic enzymes by co-substrate-mediated covalent capture

Extended Data Fig. 3

LC/ESI-MS measurements of Twin-Strep® tagged IbpA I3755C (TS-IbpA) and reactivity of β-γ non-hydrolysable TReND-6 with TS-IbpA. The mass increase of 544 Da reveals that TReND-6 coupled to IbpA and is resistant to hydrolysis even in the presence of 1 mM MgCl2. Whenever a representative result is shown, each experiment was repeated independently at least twice with similar results.

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