Extended Data Fig. 4: A reverse transcriptase for 2′-MOE RNA.

a, Chemical structure of 2′-MOE RNA. b, denaturing urea-PAGE of cDNA synthesis on 2′-MOE RNA template (sequence: 10–27 Spinraza (Supplementary Table 7)) and c, RT-PCR of cDNA synthesis. Only RT-C8 can reverse transcribe 2′-MOE RNA (as well as PS 2′-MOE RNA (Fig. 4)).