Extended Data Fig. 3: Structures of MstE in complex with different ligands.
From: A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds
Left: Side- and top-views (rotated by 90°) of MstE (a), MstE:FS-DHB (b), MstE_D109N:GG-DHB (c), MstE_D109A:MA (d), MstE_R337A:GG-DHB (e) and MstE_Y157F (f) as cartoons with helices and β-sheets colored in pink and blue, respectively. Dashed loops indicate disordered regions in the crystal structures and are labeled accordingly. Carbon atoms of ligands, if present, are shown as sticks in gold, with the DHB moieties in black. Double bonds and newly formed C-C bonds are highlighted in black. Right: Close-up views of the active sites. The 2FO-FC electron density maps (gray mesh, contoured to 1.0 σ) of ligands are shown together with residues engaged in binding. Side-views of all compounds (rotated by 90°) are depicted on the far right. Dashed lines represent important interactions. Mutated residues are colored green. Diffuse electron density in F indicates the presence of a ligand at low occupancy.
